Comparative efficacy of hydroxychloride and organic sources of zinc, copper, and manganese on egg production and concentration of trace minerals in eggs, plasma, and excreta in female broiler breeders from 42 to 63 weeks of age.

Comparative efficacy of hydroxychloride (HC) and organic (OR) sources of Zn, Cu and Mn on performance of broiler breeders (BB) between 42 and 63 weeks of age (WOA) was investigated. A total of 408 ♀ Ross 708 and 48 ♂ Yield Plus cockerels were placed in pens (17 ♀ and 2 ♂) housed in 2 rooms (12 pens/room) and allocated to one of 2 diets in a completely randomized block design (n=12). The diets had similar nutrient specifications but differed in Zn, Cu, and Mn sources: 1) HO, a blend of 80% HC and 20% OR sources, and 2) OR, 100% OR sources. Birds were fed and managed according to breeder guidelines. The egg count was recorded daily and categorized as normal or abnormal. Egg yolk color, albumen height, Haugh unit, eggshell thickness, and eggshell breaking strength were assessed every 4 wk. Individual hen body weight (BW) was recorded at 5-wk intervals to determine BW uniformity. At 52 and 63 WOA, the eggs and excreta samples were collected. At the end of the trial, 4 hens per pen were bled for plasma concentration of trace minerals and organs (liver, gizzard, spleen, kidney, and thymus) weight. There were no interactions between source and age on any parameters (P > 0.05). There were no main effects of source on egg production, eggshell quality, BW, and organs weight (P > 0.05). Hens fed HO diets had darker yolk compared to those fed OR diets (P = 0.014). The concentration of Zn in the eggs of OR BB was higher (P = 0.022) than for HO birds. However, there were no dietary effects on the concentration of trace minerals in the egg, plasma, and excreta (P > 0.05). The results indicated that a mixture of HC and O as sources for Zn, Cu, and Mn was as effective as OR sources in supporting egg production, egg quality, and trace mineral utilization in broiler breeders.

Growth performance, organs weight, intestinal histomorphology, and oocyst shedding in broiler chickens offered novel single strain Bacillus subtilis isolated from camel dung and challenged with Eimeria.

We evaluated a single strain Bacillus subtilis BS-9 direct-fed microbial (BSDFM) isolated from camel dung in Eimeria challenged broiler chickens. Seven-hundred d-old Ross 708 male chicks were placed in pens (25 birds/pen) and allocated to 2 treatments (n = 14). From d 0 to 13, control pens received untreated water (-BSDFM), and 2 treated pens received water and 2 mL x 108 colony forming unit/bird/d (+BSDFM); daily water intake (WI) was recorded. On d 9, birds in half (+Eimeria) of pens per treatment received of 1 mL of Eimeria maxima and Eimeria acervulina oocysts orally, and the other half (-Eimeria) sterile saline solution. Birds had ad libitum access to feed and a water line from d 14. Feed intake (FI), body weight (BW) and mortality were recorded for calculating BW gain (BWG) and feed conversion ratio (FCR). On d 14 and 35, samples of birds were necropsied for organ weight and intestinal measurements. Excreta samples were collected from d 14 to 19 for oocyst count. There was no treatment effect (P > 0.05) on growth performance or WI on d 0 to 9. There were interactions between BSDFM and Eimeria on d 19 (P = 0.014) and 29 (P = 0.036) BW with unchallenged +BSDFM birds being heavier than birds in the other treatments. The main effects (P < 0.05) on d 10 to 35 FI, BW, and BWG were such that +BSDFM increased and Eimeria decreased (P < 0.01) these parameters. There was interaction (P = 0.022) between BSDFM and Eimeria on d 10 to 35 FCR such that the FCR of challenged -BSDFM birds was poor than that of unchallenged counterparts, but none differed with +BSDFM birds. There was an interaction (P = 0.039) between BSDFM and Eimeria on d 14 bursa weight with challenged birds exhibiting heavier bursa than unchallenged +BSDFM birds. Eimeria reduced (P = 0.01) and BSDFM (P = 0.002) increased the villi height to crypt depth ratio. Results showed that BSDFM supplementation via water can support the growth performance of broiler chickens challenged with Eimeria and may be a strategy to reduce adverse effects of coccidiosis.

Comparative impact of bacitracin and select feed additives in the feeding program of Lohmann LSL-Lite pullets at the onset of lay through to 31 weeks of age.

There are limited investigations on the role of feed additives in easing transition of pullets to egg production phase. We investigated the effects of supplementation of bacitracin methylene disalicylate (BMD) and select feed additives (myristic acid [MA], benzoic acid [BA], and Aspergillus niger probiotic [PRO]) in feeding program for pullets from the onset of lay through to 31 weeks of age (woa). Parameters measured included hen-day egg production (HDEP), feed intake (FI), feed conversion ratio (FCR), egg quality characteristics, ceca microbial activity, apparent retention of components, and plasma metabolites. A total of 1,200 Lohmann LSL Lite pullets were procured at 18 woa and placed in enriched cages (30 birds/cage) based on body weight (BW) and allocated to five diets. The diets were a basal diet formulated to meet specifications or basal mixed with either BMD, MA, BA, or PRO. Birds had free access to feed and water throughout the experiment. Between 18 and 20 woa, birds fed BMD ate a similar (P > 0.05) amount of feed to BA birds, but more (P = 0.0003) than birds fed basal, MA, or PRO diets. Basal birds had lower HDEP (P = 0.001) and lighter eggs (P < 0.0001) than birds fed any of the feed additives between 21 and 31 woa. The basal hens had a higher (P = 0.009) abundance of Escherichia coli than birds fed BMD, BA, and PRO diets. Consequently, BMD, BA, and PRO birds had a higher (P = 0.011) Lactobacilli: E. coli ratio (LER) than hens fed the basal diet. Specifically, relative to basal-fed hens, the LER of the BMD, MA, BA, and PRO hens was higher by 37%, 21%, 26%, and 45%, respectively. Moreover, birds fed PRO tended to have a higher concentration of ceca digesta acetic acid (P = 0.072) and a lower concentration of isobutyric acid (P = 0.096). In conclusion, supplementing pullet diets with broad-spectrum antibiotics or feed additives (MA, BA, and PRO) had a positive impact on FI, and egg production linked to modulation of indices of gut health. The results suggested supplementing feed additives in feeding programs for pullets at the onset of lay can bolster productivity outcomes.

Dynamic effects of black soldier fly larvae meal on the cecal bacterial microbiota and prevalence of selected antimicrobial resistant determinants in broiler chickens.

BACKGROUND: We had earlier described the growth-promoting and -depressive effects of replacing soybean meal (SBM) with low (12.5% and 25%) and high (50% and 100%) inclusion levels of black soldier fly larvae meal (BSFLM), respectively, in Ross x Ross 708 broiler chicken diets. Herein, using 16S rRNA gene amplicon sequencing, we investigated the effects of replacing SBM with increasing inclusion levels (0-100%) of BSFLM in broiler diets on the cecal bacterial community composition at each growth phase compared to broilers fed a basal corn-SBM diet with or without the in-feed antibiotic, bacitracin methylene disalicylate (BMD). We also evaluated the impact of low (12.5% and 25%) inclusion levels of BSFLM (LIL-BSFLM) on the prevalence of selected antimicrobial resistance genes (ARGs) in litter and cecal samples from 35-day-old birds. RESULTS: Compared to a conventional SBM-based broiler chicken diet, high (50 to100%) inclusion levels of BSFLM (HIL-BSFLM) significantly altered the cecal bacterial composition and structure, whereas LIL-BSFLM had a minimal effect. Differential abundance analysis further revealed that the ceca of birds fed 100% BSFLM consistently harbored a ~ 3 log-fold higher abundance of Romboutsia and a ~ 2 log-fold lower abundance of Shuttleworthia relative to those fed a BMD-supplemented control diet at all growth phases. Transient changes in the abundance of several potentially significant bacterial genera, primarily belonging to the class Clostridia, were also observed for birds fed HIL-BSFLM. At the finisher phase, Enterococci bacteria were enriched in the ceca of chickens raised without antibiotic, regardless of the level of dietary BSFLM. Additionally, bacitracin (bcrR) and macrolide (ermB) resistance genes were found to be less abundant in the ceca of chickens fed antibiotic-free diets, including either a corn-SBM or LIL-BSFLM diet. CONCLUSIONS: Chickens fed a HIL-BSFLM presented with an imbalanced gut bacterial microbiota profile, which may be linked to the previously reported growth-depressing effects of a BSFLM diet. In contrast, LIL-BSFLM had a minimal effect on the composition of the cecal bacterial microbiota and did not enrich for selected ARGs. Thus, substitution of SBM with low levels of BSFLM in broiler diets could be a promising alternative to the antibiotic growth promoter, BMD, with the added-value of not enriching for bacitracin- and macrolide-associated ARGs.

Response of broiler breeder pullets when fed hydrolyzed whole yeast from placement to 22 wk of age.

The study examined the effects of feeding broiler breeder pullets hydrolyzed whole yeast (HY) from hatch to 22 wk of age (WOA). A total of 524-day-old Ross 708 pullets were placed in floor pens (∼24 birds/pen) for the starter (0-4 WOA) and grower (5-18 WOA) phases, then transferred to the egg production facility and redistributed to ∼20 birds/pen for the prelay phase (19-22 WOA). Two diets were allocated to pens (0-18 WOA; n = 11) and (19-22 WOA; n-12). The diets were a control corn and soybean meal diet formulated to meet specifications and control plus 0.05% HY (HY). Birds had ad libitum access to feed in the first week and daily feed allocation based on pen BW from 2 WOA. Birds had free access to water throughout the trial. Body weight (BW) and uniformity (BW CV) were monitored. Boosters for infectious bronchitis and New Castle disease vaccines were administered at 18 WOA, and samples of pullets bled for antibody titer 5-day later. One pullet/pen was randomly selected, weighed, bled for plasma biochemistry, and necropsied for organ weights, ceca digesta for short-chain fatty acids (SCFA), and leg bones morphometry. In the starter and grower phases, birds fed HY were lighter and gained less (P < 0.05) than control birds. However, there were no diet effects (P > 0.05) on growth, the BW prelay phase, or BW uniformity throughout the trial. There were no (P > 0.05) diet effects on breast, gastrointestinal, liver and bursa weights, serum antibody titers, plasma biochemistry, SCFA and bone attributes. However, pullets fed HY had heavier (P = 0.047) spleen and tended to have lower (P = 0.080) plasma concentrations of aspartate aminotransferase (AST) relative to control pullets. In conclusion, the parameters assessed showed no negative consequences of feeding HY to broiler breeder pullets. However, effects on the spleen and plasma AST may indicate modest modulation of immunity and metabolism. The impact of the provision of HY during broiler breeder pullet phase on reproductive performance and chick quality should be investigated.

Effects of pretreating wheat middlings and sunflower meal with fiber degrading enzymes on components solubilization and utilization in broiler chickens.

Pretreating fibrous feedstuffs with exogenous enzymes may improve their utilization in broiler chickens. Pretreatment of wheat middlings (WM) and sunflower meal (SM) with fiber degrading enzymes (FDE) was investigated for 1) in vitro solubilization of crude protein (CP) and fiber-degrading (experiment 1), and 2) apparent retention (AR) of CP, neutral detergent fiber (NDF), nitrogen corrected apparent metabolizable energy (AMEn), as well as the concentration of ceca digesta metabolites in broiler chickens (experiment 2). In experiment 1, WM was pretreated with FDE and SM with FDE ±â€…protease and incubated in a shaker for 24 or 48 h at 40°C and 200 rpm. Samples were centrifuged, and the supernatant used for assay of sugars and organic acids and pellet processed for determination of apparent disappearance (AD) of dry matter (DM), fiber, and CP solubilization. In experiment 2, WM and SM were pretreated with FDE for 24 h, oven-dried, and incorporated in iso-caloric and iso-nitrogenous experimental diets. Diets were: 1) a corn-soybean meal positive control (PC); 2) PC plus untreated WM and SM (negative control, NC), and diets 3, 4, 5, and 6 test diets, in which the untreated WM and SM in NC were replaced with pretreated WM and SM at 25% (N25), 50% (N50), 75% (N75), and 100% (N100), respectively. Diets were prepared in mash form in two phases (starter, days 0 to 21 and finisher, days 22 to 42) and had TiO2 (0.3%) as an indigestible marker. A total of 288 Ross708 d-old male broiler chicks were placed in cages based on body weights (6 birds/cage) and allocated diets (n = 8). Birds had free access to feed and water. Samples of excreta for AR and AMEn, and of ceca digesta for the concentration of short-chain fatty acids (SCFA) were collected at the end of each phase. Pretreatment with FDE increased (P < 0.001) solubilization of CP, AD of NDF, and release of sugars and organic acids in the supernatant. The mixture of FDE and protease further increased (P < 0.001) CP solubilization in SM. Feeding pretreated WM and SM had a linear response (P ≤ 0.038) on AMEn, and gross energy (GE) (day 21) and a quadratic response (P < 0.05) on AR of components and AMEn (day 42) and concentration of total SCFA on day 42. On day 42, N25 and N50 had higher AR of DM, CP, NDF, and GE than N75 and N100. In conclusion, pretreatment of WM and SM with enzymes increased CP and fiber degradation. Incorporating moderate amounts (N25 and N50) of pretreated WM and SM in a corn-soybean meal diet fed to broiler chickens improved nutrient and energy utilization.

Comparative growth performance, gizzard weight, ceca digesta short chain fatty acids and nutrient utilization in broiler chickens and turkey poults in response to cereal grain type, fiber level, and multienzyme supplement fed from hatch to 28 days of life.

Growth performance, gizzard weight, ceca digesta short-chain fatty acids (SCFA), and apparent retention (AR) of components were investigated in broilers and turkeys in response to cereal grain type, fiber level, and multienzyme supplement (MES) fed from hatch to 28 d of life. 480-day-old male broiler chicks and equal number of turkeys were placed separately in metabolism cages (10 birds/cage) and allocated to 8 diets. The species-specific diets were a corn or wheat-based basal diet without (LF) or with 10% corn DDGS or wheat middlings (HF) and fed without or with MES. This effectively created a 2 (grain types) × 2 (fiber levels) × 2 (MES) factorial arrangement of treatments. The diets had TiO2 as an indigestible marker. Body weight, feed intake, and mortalities were recorded to calculate body weight gain (BWG) and feed conversion ratio (FCR). Excreta samples were collected on d 25 to 27 for AR, and all birds were necropsied for gizzard weight and ceca digesta on d 28. The interaction between grain and MES in broilers was such that wheat diets with MES had the lowest (P = 0.005) FCR. In broilers, LF diets had better (P = 0.010) FCR than HF diets. The wheat diets had the highest (P = 0.006) concentration of butyric acid in broilers. Broilers fed HF and corn diets had heavier gizzard than broilers-fed LF and wheat diets. The MES improved (P < 0.05) AMEn in HF, corn, and wheat diets in broilers. The turkeys fed wheat diets had the lowest (P = 0.019) FCR. Turkeys fed HF wheat diets had the heaviest (P < 0.001) gizzard. In turkeys, the MES improved AMEn in HF and LF corn diets, and only in LF wheat diets compared to respective controls. Treatments had no effect on turkeys cecal SCFA. In conclusion, grain type, fiber, and MES did not affect growth in both species. However, species exhibited differing FCR, gizzard, and energy utilization to fiber and MES.

Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age.

Growth and response to Escherichia coli lipopolysaccharide (LPS) challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids (n-3 FA) and yeast bioactives (YB) from hatch through to 16 wk of age (woa) were investigated. Co-extruded full fat flaxseed and pulse mixture (FFF; 1:1 wt/wt) supplied n-3 FA and YB were yeast cell walls processed with ß-1,3-glucan hydrolase. A total of 1,064-day-old pullets were placed in cages (19 birds/cage) and allocated to 7 diets (n = 8). The iso-caloric and iso-nitrogenous diets were control, control + 1, 3, or 5% FFF and + 0.025, 0.05, or 0.1% YB. The birds had ad libitum access to feed and water. Body weight (BW), feed intake, and lymphoid organs weight were recorded. At 15 woa, 2 pairs of pullets/cage received intravenous injection of either 1 mL of sterile saline without or with 8 mg LPS/kg BW. Injected pullets were bled, monitored for BW and cloaca temperature at time points within 168 h. Birds fed 1% FFF were heavier (P < 0.04) than birds fed other diets at 16 woa with FFF and YB exhibiting non-linear responses. Control birds had heavier (P = 0.02) thymus at 4 woa. Spleen weight increased quadratically (P < 0.05) in response to FFF at 8 and YB at 16 woa. The LPS increased cloaca temperature and altered concentration of several plasma metabolites (P < 0.05). The interaction (P < 0.05) between LPS and diet was such that control birds exhibited lower creatine kinase (CK) upon challenge with LPS relative to birds fed other diets. The LPS birds fed 1% FFF and 0.05% YB showed higher plasma albumin than non-LPS cohorts. Non-LPS birds fed control, 1 and 3% FFF had higher plasma K than LPS cohorts. In general, FFF and YB exhibited linear and quadratic effects (P < 0.05) on select plasma metabolites. In conclusion, dietary provision of n-3 FA and YB influenced pullet BW at sexual maturity, development of lymphoid organs and modulated some plasma metabolites in response to LPS.

Egg production, egg quality, and fatty acids profiles in eggs and tissues in Lohmann LSL lite hens fed algal oils rich in docosahexaenoic acid (DHA).

Enriching eggs with omega-3 fatty acids (n-3 FA), such as docosahexaenoic acid (DHA), is a well-accepted practice that benefits the egg industry and consumers. However, issues around cost, sustainability, and product acceptance have necessitated the search for alternatives to feeding hens fish oil for DHA enrichment. The effects of feeding 2 algal oils on egg production and DHA enrichment in eggs and selected tissues were investigated. The algal oils were: 1) OmegaPro (OPAO) standardized algal oil for DHA content and 2) Crude algal oil (CAO). A total of 400, 46-wk-old Lohmann LSL lite hens were housed in enriched cages (10 birds/cage) and allocated 5 diets (n = 8) for a 12-wk trial. The iso-caloric and -nitrogenous diets were a standard corn and soybean meal diet, standard plus 0.25 or 0.76% OPAO and standard plus 0.23 or 0.69% CAO; algal oils diets supplied similar DHA at each level. Egg production indices (hen day egg production, feed intake, FCR, egg weight, egg mass, and eggshell quality) were monitored for 10 wk. Diet samples were analyzed for fatty acids (FA) on wk 1, 6, and 12 and eggs on wk 4, 5, 6, 9, and 12. At the end of the trial, one hen/cage was weighed and dissected for liver, breast and thigh for FA and long bones for ash content analyses. Concentration of omega-6 to omega-3 FA ratio was 12.9, 6.64, 3.48, 6.96, and 3.59 for standard, 0.23 and 0.76% OPAO, 0.25 and 0.69% CAO, respectively. Algal oils increased (P ≤ 0.046) eggshell thickness linearly. The concentration of DHA in the eggs from the birds fed the standard, 0.23 and 0.76% OPAO, 0.25 and 0.69% CAO was 84, 195, 286, 183, and 297 mg/100g egg, respectively, and algal oils enriched eggs with DHA linearly and quadratically (P ≤ 0.01). In conclusion, algal oils increased the concentration of DHA in eggs and had no adverse effects on egg production and eggshell quality.

Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs.

In this study, we assessed the efficacy of a novel Bacillus subtilis probiotic in improving growth performance and gut responses in comparison to pharmacological zinc oxide (ZnO) in nursery pigs. A total of 96 piglets were randomly assigned to four groups: Negative control (NC), Positive control (PC, 3000 mg Zn /kg feed), B.subtilis low dose (BS9-L, 2 × 107 CFU/pig) and B.subtilis high dose (BS9-H, 2 × 109 CFU/pig). Growth performance, diarrhea rate, gut mucosal gene expression and fecal microbial populations were evaluated. B.subtilis administration did not improve piglet bodyweight. BS9-L showed (P < 0.05) higher average daily gain (ADG) in Period 2 (D14-D28). BS9 groups had (P < 0.001) lower feed conversion ratio (FCR) in Period 2 (D14-D28) and overall. Like the ZnO-group, BS9 groups had lower (P < 0.01) diarrhea rate. A significant reduction (P < 0.05) in fecal E. coli, total coliforms, and an increase in lactic acid bacteria and Bacillus spp. in BS9 groups was observed. BS9 group had reduced (P < 0.05) mRNA levels of intestinal IL-8 and higher levels of MUC-1 and occludin and TJP-1 compared to negative control. These findings suggest that probiotic BS9, may promote growth performance, and ameliorate various indicators of intestinal health in piglets. Hence, it may serve as a prospective alternative to ZnO growth promoter in commercial swine production.

Growth performance, organ weight, and plasma metabolites in broiler chickens fed corn-soybean meal diet containing berry pomaces and fed without or with multienzymes supplement.

This study evaluated effects of feeding low-bush wild blueberry (LBP) and organic American cranberry (CRP) pomaces without or with multienzyme supplement (ENZ) on growth performance, organ weight and plasma metabolites in broiler chickens. Nonenzyme-fed (no-ENZ: n = 1,575) and enzyme-fed (ENZ: n = 1,575) day-old male Cobb500 broilers were placed in floor pens (45 chicks/pen) and allocated to five corn-soybean meal-based diets: a basal diet supplemented with either bacitracin methylene disalicylate (BMD, 55 mg/kg), 0.5 or 1% of CRP or LBP in a 2 × 5 factorial design for 35-day experiment. Body weight (BW), feed intake (FI) and mortality were recorded whereas BW gain (BWG) and feed conversion ratio (FCR) were calculated. Birds were sampled at days 21 and 35 for organ weights and plasma metabolites. There were no interactions between diet and ENZ on any parameter (P > 0.05) and no effect of ENZ on overall (d 0-35) growth performance and organ weights (P > 0.05). Birds fed BMD were heavier (P < 0.05) at d 35 and had better overall FCR than berry-supplemented birds. Birds fed 1% LBP had poor FCR than birds fed 0.5% CRP. Birds fed LBP exhibited heavier liver (P < 0.05) than birds fed BMD or 1% CRP. The highest plasma concentrations of aspartate transaminase (AST), creatine kinase (CK) at d 28 and gamma-glutamyl transferase (GGT) at d 35 were found in ENZ-fed birds (P < 0.05). At d 28, birds fed 0.5% LBP showed higher plasma AST and CK concentrations (P < 0.05). However, CRP feeding resulted in a lower plasma CK level compared with BMD feeding (P < 0.05). The lowest cholesterol level was detected in 1% CRP-fed birds. In conclusion, this study showed no ENZ effects to potentiate berry pomaces on the overall growth performance of broilers (P < 0.05. However, plasma profiles revealed the potential of ENZ to modulate the metabolism of pomace-fed broilers. LBP increased BW during the starter phase, while CRP increased BW during the grower phase.

Effects of partial or complete replacement of soybean meal with commercial black soldier fly larvae (Hermetia illucens) meal on growth performance, cecal short chain fatty acids, and excreta metabolome of broiler chickens.

Black soldier fly larvae meal (BSFLM) is receiving great attention as a rich source of protein and antimicrobials for poultry. Therefore, we evaluated the effects of partially or completely replacing soybean meal (SBM) with commercial BSFLM on growth performance, tibia traits, cecal short chain fatty acid (SCFA) concentrations, and excreta metabolomes in broiler chickens (Gallus gallus domesticus). A total of 480 day-old male Ross × Ross 708 chicks were assigned to 6 diets (8 replicates/diet): a basal corn-SBM diet with in-feed bacitracin methylene disalicylate (BMD), a corn-SBM diet without BMD (0% BSFLM), and four diets in which the SBM was substituted with 12.5, 25, 50, and 100% BSFLM. Body weight (BW), feed intake (FI) and cumulative feed conversion ratio (cFCR) were monitored on days 14, 28, and 35. Cecal SCFA levels were determined on days 14, 28, and 35. Tibia traits and excreta metabolomes were determined on day (d) 35. On d14, birds fed 12.5 and 25% BSFLM had a similar BW, FI, and cFCR as birds fed BMD (P > 0.05). On d 35, birds fed 12.5% BSFLM had a similar BW, FI and cFCR as birds fed BMD or 0% BSFLM (P > 0.05). For each phase, birds fed 100% BSFLM had a lower BW, FI and higher cFCR than birds fed BMD or 0% BSFLM (P < 0.05). On d 35, BW decreased linearly, quadratically, and cubically with increasing levels of BSFLM (P < 0.01). Overall (d 0-35), BSFLM linearly, quadratically, and cubically decreased FI and quadratically and cubically increased cFCR (P < 0.01). Quadratic responses were observed for tibia fresh weight (P = 0.049) and ash content (P = 0.022). BSFLM did not impact cecal SCFAs levels. The excreta metabolome of birds fed 100% BSFLM clustered independently from all other groups and exhibited greater levels of putatively identified methionine, lysine, valine, glutamine, histidine and lower levels of arginine as compared to all diets. Taken together, substitution of SBM with ≤25% of BSFLM in the starter phase may be used as an alternative to BMD.

Research Note: Impact of Eimeria on apparent retention of components and metabolizable energy in broiler chickens fed single or mixture of feed ingredients-based diets.

The effect of Eimeria on apparent retention (AR) of components and metabolizable energy corrected for nitrogen (AMEn) content in corn, wheat, soybean meal (SBM), and pork meal (PM) was investigated in broiler chickens. A total of 840 male d-old Ross 708 chicks were placed in 84 cages (10 birds/cage) and allocated either a nitrogen-free diet (NFD), or 1 of 6 test cornstarch-based semipurified diets: 1) corn, 2) wheat, 3) SBM, 4) PM, 5) corn, SBM, and PM (CSP) mixture, and 6) wheat, SBM, and PM (WSP) mixture (n = 12). Diets contained 0.3% titanium dioxide and nutrient digestibility was determined by difference method using NFD. On d 10, birds in half of replicates per diet were orally challenge with 1 mL of E. acervulina and E. maxima culture and the other half equal volume of saline. Excreta samples were collected from d 12 to 14. With exception of AR of Ca and P, there was no interaction (P > 0.05) between Eimeria and diet on AR of dry matter, crude fat (CF), crude protein and gross energy and AMEn of ingredients. Eimeria reduced AR of CF (P = 0.01) and had a tendency to reduce AR of DM (P = 0.09) and AMEn (P = 0.063) of ingredients. The data demonstrated exposure to Eimeria impacted nutrient retention and energy utilization irrespective to diet composition.

Egg production, egg quality, organ weight, bone ash, and plasma metabolites in 30-week-old Lohmann LSL lite hens fed corn and soybean meal-based diets supplemented with enzymatically treated yeast.

Highly prolific modern hens are susceptible to metabolic disorders that could be modulated by functional feedstuffs such as enzymatically treated yeast (ETY). Therefore, we assessed the dose-response of ETY on hen-day egg production (HDEP), egg quality attributes, organ weight, bone ash, and plasma metabolites in laying hens. A total of 160 thirty-week-old Lohmann LSL lite hens were placed in 40 enriched cages (4 birds/cage) based on body weight (BW) and allocated to 5 diets in a completely randomized design for a 12-wk trial. The diets were isocaloric and isonitrogenous corn and soybean meal based supplemented with 0.0, 0.025, 0.05, 0.1, or 0.2% ETY. Feed and water were provided ad libitum; HDEP and feed intake (FI) were monitored weekly, whereas egg components, eggshell breaking strength (ESBS), and thickness (EST) were monitored biweekly, and albumen IgA concentration was measured on wk 12. At the end of the trial, 2 birds/cage were bled for plasma and necropsied for liver, spleen, and bursa weight, ceca digesta for short chain fatty acids (SCFA) and tibia and femur for ash content. Supplemental ETY reduced HDEP quadratically (P = 0.03); the HDEP was 98, 98, 96, 95, and 94% for 0.0, 0.025, 0.05, 0.1, and 0.2% ETY, respectively. However, ETY linearly and quadratically (P = 0.01) increased egg weight (EW) and egg mass (EM). Specifically, EM was 57.9, 60.9, 59.9, 58.9, and 59.2 g/b for 0.0, 0.025, 0.05, 0.1, and 0.2% ETY, respectively. Egg albumen increased linearly (P = 0.01), and egg yolk decreased linearly (P = 0.03) in response to ETY. In response to ETY, the ESBS and plasma Ca increased linearly and quadratically (P ≤ 0.03). Plasma concentration of total protein and albumin increased quadratically (P ≤ 0.05) with ETY. Diets had no (P > 0.05) effects on FI, FCR, bone ash, SCFA, and IgA. In conclusion, 0.1% or higher ETY reduced egg production rate; however, linear improvement in EW and shell quality linked to larger albumen and higher plasma protein and Ca suggested modulation in protein and calcium metabolism.

Complete replacement of soybean meal with black soldier fly larvae meal in feeding program for broiler chickens from placement through to 49 days of age reduced growth performance and altered organs morphology.

Black soldier fly larvae meal (BSFLM) is characterized with good nutritional and functional attributes. However, there is limited data on inclusion of BSFLM in broiler chicken rations from placement through to market weight. Therefore, we examined growth and organ responses of partial to complete replacement of soybean meal (SBM) with BSFLM in a practical feeding program. A total of 1,152 d-old male Ross × Ross 708 chicks were allocated to 48 pens and assigned one of six diets (n = 8). The diets were: a basal corn-SBM diet (0%BSFLM), 4 diets in which SBM in 0%BSFLM was replaced with BSFLM at 12.5, 25, 50, and 100% and a final diet (0 + AGP) in which 0%BSFLM was treated with coccidiostat (70 mg Narasin/kg) and antibiotic (55 mg Bacitracin Methylene Disalicylate/kg). For energy fortification, soy oil was used for 0%BSFLM diets and black soldier fly oil in the other diets. Body weight, feed intake (FI), BW gain (BWG), and mortality-corrected feed conversion ratio (FCR) were reported. Organ weights were recorded on d 24 and 49. On d 10, birds fed diets 12.5, 25, and 0 + AGP had higher BWG than birds fed diets 0, 50, and 100 (P < 0.01), and birds fed diet 100 had lower BWG than birds fed diets 0 or 50 (P < 0.01). Birds fed diets 50 and 100 had lower BWG than birds fed all other diets on d 24 and 49 (P < 0.05). Overall (d 0-49), BSFLM linearly (P < 0.01) decreased BW, BWG, and FI and increased FCR and mortality. The overall BWG of 50 and 100% BSFLM birds was 92 and 81% of birds fed 0%BSFLM, repectively and coresponding overall FI was 96 and 90%. An increase in gizzard, small intestine, pancreas, and liver relative weights were observed with increasing BSFLM inclusion (P < 0.01). The data indicated that lower levels of BSFLM could provide some growth-promoting effects commensurate to antibiotics in the starter phase. However, replacing SBM with greater amounts (≥50) of BSFLM reduced growth and increased organ size.

Evaluation of standardized ileal digestibility of amino acids and metabolic availability of methionine, using the indicator amino acid oxidation method, in black soldier fly larvae (Hermetia illucens) meal fed to growing pigs.

Standardized ileal digestibility (SID, %) of crude protein (CP) and amino acid (AA) and the metabolic availability (MA) of Met using the indicator amino acid oxidation (IAAO) method, in partially defatted black soldier fly larvae (PD-BSFL) meal were determined in growing pigs in 2 experiments. The Met SID value was then compared numerically with the Met MA to understand how different SID is compared with its MA value. In Exp. 1, 6 ileal-cannulated barrows (initial body weight [BW] = 18.03 ± 0.34 kg) were used in a 2-period switch back design and fed either a nitrogen-free diet (NFD) or test diet, with PD-BSFL meal as the sole source of AA, over two 11-d experimental periods, at a feeding level of 2.8 × estimated maintenance digestible energy requirement. Barrows were adapted for 9-d to the diet, followed by continuous 8-h ileal digesta collection on day 10 and 11. Digesta were pooled per pig within period. The SID of CP and Met of PD-BSFL meal were 76.1 ± 6.2% and 90.4 ± 3.9%, respectively. In Exp. 2, 7 barrows (initial BW = 18.77 ± 0.69 kg) were used in a 7 × 7 Latin square design with L-[1-13C]-Phe as the indicator AA. Each pig was randomly assigned to 1 of 7 dietary treatments over seven 3-d experimental periods. Two diet types were studied including reference (crystalline AA) and PD-BSFL test diets, each supplying graded intakes of Met at 55, 65, and 75% of the estimated SID requirement (NRC, 2012). The MA of Met was determined by comparing the IAAO response between the reference and PD-BSFL test diet using the slope-ratio method. Linear regression determined a negative slope of the best fit line for both the reference and test diets (P < 0.05). The MA of Met in PD-BSFL meal was 53.3%, which is as expected lower than the SID value. While it is generally appreciated that MA will be less than SID, the use of SID is more practical. In cases where SID cannot explain physiological outcomes of feeding a novel ingredient, IAAO may provide additional insight into whether MA should be explored.

The interest in black soldier fly larvae (BSFL) meal as a protein ingredient in swine feed has grown in the past years. As a novel protein ingredient, it is beneficial to evaluate the amino acid (AA) digestibility and metabolic availability (MA) of the limiting AA, Met, in pigs, in BSFL meal prior to incorporation in feed for a more precise formulation. Two different methodologies were used to determine the AA digestibility and MA, standardized ileal digestibility (SID) and indicator amino acid oxidation (IAAO) method, respectively. Based on the SID values, the AA digestibility of BSFL meal, for some, but not all AA, is comparable to other commonly used protein ingredients in commercial swine feed. When compared with the MA result of Met, the Met SID value is much lower. This indicates that not all digested Met is available for protein synthesis or other metabolic processes in the animal.

Comparative protein quality in black soldier fly larvae meal vs. soybean meal and fish meal using classical protein efficiency ratio (PER) chick growth assay model.

Black soldier fly larvae meal (BSFLM) is a relatively new ingredient that has attracted attention for application in human, farm, and companion animal nutrition. However, there is limited data on protein quality (PQ) of BSFLM. This study compared PQ in BSFLM with soybean meal (SBM) and fishmeal (FM) using the classical protein efficiency ratio (PER) chick growth assay model. A total of 240-male day-old Ross × Ross broiler chicks were allocated to 6 test diets for 10 d test. The corn starch-dextrose based diets were: 5 test diets containing 10% crude protein (CP) from casein (as a standard assay diet), SBM, FM, or BSFLM without or with (BSFLM+) additional essential amino acids (EAA) to match the levels of the SBM diet on digestible basis. A sixth diet was nitrogen-free (NFD), to enable calculation of net protein ratio (NPR). The birds had ad libitum access to feed and water, body weight (BW) and feed intake (FI) were recorded at the beginning and end of the experimental feeding period. Body weight gain (BWG), gain to feed ratio (G:F), crude protein intake (CPI), PER, and NPR were calculated. The analyzed CP (as fed) was 10.1, 12.8, 9.5, 11.7, and 14.9% for casein, SBM, FM, BSFLM, and BSFLM+ diets, respectively. Birds fed BSFLM+ had greater BWG (P < 0.01) than birds fed other diets. Feed intake was greater (P < 0.01) for birds fed BSFLM+ and FM diets than birds fed other diets. Birds fed BSFLM+ ate (P < 0.01) more CP than birds fed other diets. Diets had no (P > 0.05) effects on PER and NPR. In terms of ranking, the PER was 2.44, 2.38, 2.34, 2.28, and 2.10 g BWG/g CPI for BSFLM+, SBM, FM, casein, and BSLM, respectively. Corresponding values for NPR were 2.69, 2.78, 2.70, 2.74, and 2.48 g BWG/g CPI, respectively. In conclusion, the protein quality of BSFLM without or with additional EAA was comparable with FM and SBM.

Production systems and important antimicrobial resistant-pathogenic bacteria in poultry: a review.

Economic losses and market constraints caused by bacterial diseases such as colibacillosis due to avian pathogenic Escherichia coli and necrotic enteritis due to Clostridium perfringens remain major problems for poultry producers, despite substantial efforts in prevention and control. Antibiotics have been used not only for the treatment and prevention of such diseases, but also for growth promotion. Consequently, these practices have been linked to the selection and spread of antimicrobial resistant bacteria which constitute a significant global threat to humans, animals, and the environment. To break down the antimicrobial resistance (AMR), poultry producers are restricting the antimicrobial use (AMU) while adopting the antibiotic-free (ABF) and organic production practices to satisfy consumers' demands. However, it is not well understood how ABF and organic poultry production practices influence AMR profiles in the poultry gut microbiome. Various Gram-negative (Salmonella enterica serovars, Campylobacter jejuni/coli, E. coli) and Gram-positive (Enterococcus spp., Staphylococcus spp. and C. perfringens) bacteria harboring multiple AMR determinants have been reported in poultry including organically- and ABF-raised chickens. In this review, we discussed major poultry production systems (conventional, ABF and organic) and their impacts on AMR in some potential pathogenic Gram-negative and Gram-positive bacteria which could allow identifying issues and opportunities to develop efficient and safe production practices in controlling pathogens.

Enzymatically treated yeast bolstered growth performance of broiler chicks from young broiler breeders linked to improved indices of intestinal function, integrity, and immunity.

Older breeder chicks (OBC) are heavier and robust at hatch than younger breeder chicks (YBC). However, the implications of broiler breeder age on chick intestinal function and the role of functional feedstuffs are unexplored. We evaluated the effects of broiler breeder age and the impact of feeding YBC enzymatically treated yeast on growth, nutrient utilization, and indices of intestinal function. Fertile Ross 708 eggs: 2,250 (56.5 ± 3.4g) from 30-wk-old (YBC) and 550 (64.2 ± 4.2 g) from 47-wk-old (OBC) were hatched and placed in 48 pens (44 chicks/pen) containing equal males and females for growth and intestinal function evaluation and 36 cages (5 chicks/cage) for metabolizable energy (AME). Five corn and soybean meal-based diets were formulated to contain 0, 0.05, 0.10, 0.20, and 0.40% HY40 for a 3-phase feeding program (starter: days 0-10, grower: days 11-24, and finisher; days 25-42). Grower phase diets also contained a 0.3% TiO2 indigestible marker. The diets were allocated within YBC in a completely randomized block design (n = 8 for pens; n = 6 for cages). The OBC were fed a 0% yeast diet. Feed and water were provided freely; BW and feed intake were monitored, and excreta samples were collected on days 17 to 21 for apparent retention (AR). Birds were necropsied for plasma, jejunal tissues, organs weight, and ceca digesta. The OBC were heavier (P < 0.01) than YBC at hatch. Final BW of OBC and YBC fed, ≥0.10% yeast, was similar (P > 0.05). The OBC had similar FCR (P > 0.05) to YBC fed 0 to 0.10% yeast but higher (P = 0.003) than for YBC fed ≥0.20% yeast. Jejunal villi height to crypt depth ratio (VCR) and IgA were higher in OBC than 0% yeast (P = 0.01). Yeast increased VCR, bursa weight, jejunal, and plasma IgA (P = 0.01). The YBC fed ≥0.10% yeast had higher (P < 0.05) AR of crude protein, and gross energy than OBC and YBC fed 0 or 0.05% yeast. In conclusion, yeast improved YBC performance to the level of OBC linked to improved intestinal function, integrity, and immunity.

Physical Treatment Reduces Trypsin Inhibitor Activity and Modifies Chemical Composition of Marama Bean (Tylosema esculentum).

The utility of the marama bean (MB) as an alternative protein source to soybean (SB) can be limited by the high concentration of trypsin inhibitors (TI). The physical treatment of MB has the potential to ameliorate the antinutritional activities of TI and modify other chemical components. Thus, this study investigated the effects of physical treatments on the chemical components and trypsin inhibitor activity (TIA) of raw MB and SB. The bean substrates were subjected to each of the following treatment methods: (1) room temperature (20−22 °C) soaking for 24 h; (2) electric stove cooking at 100 °C for 10, 20, and 30 min; (3) steam autoclaving at a temperature of 110 °C and pressure of 7 pounds per square inch (psi), as well as a temperature of 121 °C and 7 psi for 5, 15, and 30 min; (4) pre-soaked autoclaving at 110 °C (7 psi) and 121 °C (17 psi) for 5, 15, and 30 min. Treated MB and SB had greater (p < 0.05) crude protein content than untreated samples. All the treatments (except 24 h soaking of MB) reduced (p < 0.05) the TIA and ash content. Marama and SB are similar in protein content, but their amino acids profile and TIA are quite different. Soaking for 24 h was less effective in reducing TIA in MB and SB, compared to the thermal methods, and it was detrimental to the ash and amino acids profile of the two beans. Soaking prior to autoclaving yielded beans with the lowest TI concentrations. In conclusion, thermal methods reduced the TI contents and modified the level of proximate components and amino acids profile of the beans.